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Lyme Disease Message Board


Lyme Disease Board Index


Test Name: IFA B Burgdorferi G/M/A Result: <1:40 TITER

Ranges:
<1:40 Negative
1:40 Inderterminate
= OR >1:80 Positive





Lyme IgM WESTERN BLOT

IGenex Interpretation is based on internal validation stugies. By IGenex criteria, IgM WB is considered positive if two or more of the double starred bands are present. The Igm WB is considered negative if less than 2 starred bands are present. A positive suggests exposure to B burgdorferi. By CDC/NYS criteria, Igm WB is negative if less than 2 bands are present.
LIMITATION: Positive result for 31 and/or 34kda may be present after vaccination in uninfected persons. Infection with HSV, EBV, HCV and/or syphillis (RPR+) may give false (+) results. In a sample set of 165 well characterized specimens by IGenex criteria; and 99% and 58% by CDC/NYS criteria respectively.
****PRESENCE OF ONLY ONE DOUBLE STARRED BAND OR INDETERMINATE DOUBLE STARRED BANDS IN A NEGATIVE REPORT MAY INDICATE CLINICAL SIGNIFICANCE**** THEREFORE, WE RECOMMEND TESTING WITH ANOTHER METHOD AND/OR RESTEST IN 4-6WEEKS.
BAND INTENSITY: Negative (-) : No visible band present. Indeterminate (IND) : Band present with intensity < Weak (1+) Positive Control. Positive (1+ to 4+) : Band present at an intensity >/= Weak (1+) Positive Control.


IGM Result NEGATIVE
CDC/NYS Result NEGATIVE

18 kda. -
22 kda. -
**23-25 kda. -
28 kda. -
30 kda. -
**31 kda. IND
**34 kda. -
**39 kda. -
**41 kda. IND
45 kda. -
58 kda. -
66 kda. -
73 kda. -
**83-93 kda. -




LYME IgG WESTERN BLOT

IGenex interpretation is based on internal validation studies. By IGenex criteria, IgG WB is reported positive if two or more of the double starred bands below are present. IgG WB is negative is lees than 2 starred bands are present.
A positive result suggests exposure to B burgdorferi.
By CDC/NYS criteria, IgG WB is reported positive if 5 of the following bands are present: 18,23-25,28,30,39,41,45,58,66,83-93kda. The IgG WB is nagtive if less than 5 bands are present.
LIMITATION: Positive result for 31 and/or 34kda may be present after vaccination in uninfected persons. In a sample set of 165 well characterized specimens with 36% positivity rate, the assay specificty and sensitivity was 96% & 63% by IGenex criteria; 100% and 38% by CDC/NYS criteria respectively.
****PRESENCE OF ONLY ONE DOUBLE STARRED BAND OR INDETERMINATE DOUBLE STARRED BANDS IN A NEGATIVE REPORT MAY INDICATE CLINICAL SIGNIFICANCE**** THEREFORE, WE RECOMMEND TESTING WITH ANOTHER METHOD AND/OR RESTEST IN 4-6WEEKS.
BAND INTENSITY: Negative (-) : No visible band present. Indeterminate (IND) : Band present with intensity < Weak (1+) Positive Control. Positive (1+ to 4+) : Band present at an intensity >/= Weak (1+) Positive Control.

Igenex - IGG - Result Negative
CDC/NYS- Result Negative

18 kda -
22 kda -
*23-25 kda -
28 kda -
30 kda -
**31 kda -
**34 kda -
**39 kda -
**41 kda +
45 kda -
58 kda -
66 kda -
73 kda -
**83-93 kda -


Whole Blood
Genomic or Plasmid B burg NEGATIVE
Plasmid - B burgdorferi NEGATIVE

Serum
Genomic or Plasmid B burg NEGATIVE
Plasmid - B burgdorferi POSITIVE -Confirmed by Southern Dot Blot


Any help would be greatly appreciated. Thanks.





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